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Hands on Assisted Reproduction (IVF/ICSI)

Module: 5A
Course: IVF / ICSI / Micromanipulation

Duration: 80 hours (10 days)
Fees: USD 7,950.00

Course objectives:

This course provides a one-on-one comprehensive training in the clinical management and laboratory practices of IVF and ICSI. The syllabus covers the following areas:

Laboratory Training:


Supplies & Culture Medium

IVF Techniques

Advanced ART Techniques

Pre-Implantation Genetic Diagnosis

  • Blastomere biopsy:
    • Learn the setting up of the micromanipulator
    • Biopsy of mouse/hamster 8-cell stage embryos
    • Use of laser and acid Tyrode's for zona drilling
    • Blastomere and polar body aspiration and fixation techniques
  • Cell spreading
  • Fixation techniques


Andrology Techniques

Sample Course Schedule for Module 5A


  • Introduction to embryology and assisted reproduction techniques
  • Embryo selection
  • Optimization of embryo culture conditions
  • Essential instruments and disposable supplies for an IVF laboratory
  • Extended culture and single embryo transfer
  • Embryo evaluation, grading and assisted hatching
  • Designing of new ART Laboratory:
    Setting up of an ideal laboratory is the key to success in IVF. Learn from our embryologists and scientists the key factors to be kept in mind while setting up a state-of-the-art IVF lab
  • Optimization of IVF lab procedures (quality control, quality assurance, proficiency testing, accreditation and documentation)
  • Sperm preparation techniques:
    Sperm processing technique is vital in an era of increasing male factor infertility. The level of reactive oxygen species and DNA damage is known to increase by iatrogenic damage during faulty sperm preparation techniques, thereby affecting ART outcomes. Learn from our experts the ideal sperm processing techniques.
  • Sperm selection
    Learn the latest sperm selection techniques for ICSI sperm (discussion).
  • Ovulation induction protocols
    The success of IVF mainly depends on the endocrine milieu created by the reproductive endocrinologist for super ovulation. Discuss with specialists the pros and cons of different stimulation protocols.
  • Oocyte retrieval

Module: 5B
Course: ICSI / Micromanipulation

Duration: 40 hours (5 days)
Fees: USD 6,200.00

Course objectives:

This course provides a one-on-one training to develop knowledge and skills in all aspects of micromanipulation for ICSI. The syllabus covers the following areas:

Laboratory Training:


IVF Techniques

Clinical Observation in an IVF Lab (Optional)*:

  • Logistics of running an IVF Lab
  • Observing IVF/ ICSI procedures

* Extra fees will apply

Sample Course Schedule for Module 5B


ICSI Videos

Video 1

Video 2

Video 3

(Please click on a link above, to open the video clip with the media player, supported by your browser. Alternatively, you may 'Right Click' on a link and choose an action.)


Module: 5C
Course: Advanced ART Techniques

Duration: 40 hours (5 days)
Fees: USD 6,850.00

Course objectives:

This course provides a one-on-one advanced training in high complexity techniques in a Laboratory. The syllabus covers the following areas:

Laboratory training:

  • Assisted hatching (chemical)
    Learn how to perform assisted hatching using the chemical technique (Tyrode's solution)
  • Embryo biopsy
    Learn how to perform the embryo biopsy by removing one or more blastomeres. This technique is needed for laboratories which intend to perform pre-implantation genetic diagnosis
  • Embryo cryopreservation
    Learn different protocols of embryo freezing , for either embryos with 8 or less cells or in blastocyst stage
  • ICSI with micro-HOS test
    Learn how to perform the micro hypo osmotic swelling test for semen samples with no motility (practical)
Sample Course Schedule for Module 5C

Module: 5D
Course: Customized ART Training

Duration: Selected by Trainee
Fees: To Be Determined

Course objectives:

This course provides customized training in the clinical management and laboratory practices of IVF and ICSI.

How Module 5D Works:

  1. Candidates select
    1. the techniques they wish to learn (Modules 1-8);
    2. the duration of their training (1- 8 weeks); and
    3. whether they desire clinical observation in an offsite IVF laboratory
  2. Candidates consult with the Course Director regarding their training needs and choices. The candidate and the Course Director in consultation will decide on the appropriate modules and duration of training required by the candidate
  3. A personalized training schedule is created specifically for the candidate
  4. Training is provided at a pace established by the candidate and is geared towards fulfilling the candidates personal needs
Sample Course Schedule for Module 5D


Hands on Assisted Reproduction (IVF/ICSI)



Training in this module accredited by the
American College of Embryology –
Click on the logo for details

Click to enlarge

Educational teaching methods:

  • Interactive discussion and teaching sessions
  • Hands-on laboratory training sessions
  • Live lectures and multimedia presentations

Evaluation of trainee:

  • Knowledge/Theoretical Component (30%): Multiple choice questions on lab techniques linked to each course module
  • Practical Skills (50%): Tested on specific lab techniques taught during the training
  • Verbal Communication & Comprehension Skills / Integrative Component (20%): PowerPoint talk on a topic linked to a course module and viva voce
  • The performance of the candidate throughout the course will be reflected in a Grading Report
  • Candidates who meet the training requirements and successfully complete the course work will be awarded the Training Certificate

Each participant will receive:

  • Course Syllabus with Reading Material and Lab Protocols
  • Each participant will receive a complimentary hardcopy of the Practical Manual of In Vitro Fertilization (Editors: Nagy, Z., Varghese, A., and Agarwal, A.), Publisher: Springer (Value of $219)
  • Free Access to the Cleveland Clinic Alumni Library
  • WHO Laboratory Manual for the Examination and Processing of Human Semen. 5th Edition, 2010 (Digital file)
  • Certificate of Training (issued upon successful assessment by the instructor) and Grading Report
  • Daily Meal (Lunch) Tickets

Virtual 360° Tour of the American Center for Reproductive Medicine Research Laboratories and Andrology Center

Course Evaluation by Past Trainees

Testimonials by Past Trainees

Contact the Course Director
Dr. Ashok Agarwal

Professor, Lerner College of Medicine,
Case Western Reserve University
Director, Andrology Center
Director, American Center for Reproductive Medicine
Staff, Glickman Urological & Kidney Institute, and
Ob-Gyn and Women's Health Institute,
Anatomic Pathology, and Immunology

Cleveland Clinic
10681 Carnegie Ave., X11
Cleveland, Ohio 44195 USA

Tel: 216-444-9485
Fax: 216-445-6049

ResearchGate Profile
LinkedIn Profile
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Dr. Agarwal's Twitter: @AshokAgarwalCCF
ACRM's Twitter: @CleClinReproMed
ACRM's Facebook: Cleveland Clinic Center for Reproductive Medicine