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Hands on Assisted Reproduction (IVF/ICSI):

 

Module 5 A


 

Module 5 B


 
 

Course objectives:

This course provides a one-on-one comprehensive training in the clinical management and laboratory practices of IVF and ICSI. The syllabus covers the following areas:

Laboratory Training:

Equipments

Supplies & Culture Medium

IVF Techniques

Advanced ART Techniques

Pre-Implantation Genetic Diagnosis

  • Blastomere biopsy:
    Learn the setting up of the micromanipulator
    Biopsy of mouse/hamster 8-cell stage embryos
    Use of laser and acid Tyrode's for zona drilling
    Blastomere and polar body aspiration and fixation techniques
  • Cell spreading
  • Fixation techniques

Cryopreservation

Andrology Techniques

Clinical Observation in an IVF Lab*:

  • Logistics of running an IVF Lab
  • Observing IVF/ ICSI procedures
  • Oocyte retrieval
  • Oocyte assessment
  • Fertilization check
  • Embryo assessment
  • Intrauterine insemination

*Clinical rotation in an IVF laboratory is optional. Extra fees will apply.

Discussion:

  • Equipments and supplies used in an IVF laboratory
  • Designing of new ART Laboratory:
    Setting up of an ideal laboratory is the key to success in IVF. Learn from our embryologists and scientists the key factors to be kept in mind while setting up a state-of-the-art IVF lab.
  • Optimization of IVF lab procedures (quality control, quality assurance, proficiency testing, accreditation and documentation)
  • Sperm preparation techniques:
    Sperm processing technique is vital in an era of increasing male factor infertility. The level of reactive oxygen species and DNA damage might increase by faulty sperm preparation techniques thereby affecting the ART outcomes. Learn from our experts the ideal sperm processing techniques.
  • Sperm selection
    Learn the latest sperm selection techniques for ICSI sperm (discussion).
  • Ovulation induction protocols
    The success of IVF mainly depends on the endocrine milieu created by the reproductive endocrinologist for super ovulation. Discuss with specialists the pros and cons of different stimulation protocols.
  • Ultrasound:
    Follicle aspiration using sonography is the vital step to obtain the mature oocytes. Learn from the experts the various trouble shooting points while follicle aspiration/follicle monitoring during ovulation induction.
  • Oocyte retrieval

Course duration:

Participants receive 2 weeks (80 hours) of instructions in the following areas:

  • Lectures, discussions and PPT - 20 hours
  • Laboratory training sessions - 60 hours
 

Course objectives:

This course provides a one-on-one training to develop knowledge and skills in all aspects of micromanipulation for ICSI. The syllabus covers the following areas:

Laboratory Training:

Equipments

IVF Techniques

Clinical Observation in an IVF Lab*:

  • Logistics of running an IVF Lab
  • Observing IVF/ ICSI procedures

Course duration:

Participants receive 5 days (40 hours) of instructions in the following areas:

  • Interactive discussions and observation - 15 hours
  • Laboratory training sessions - 25 hours

 
 
IVF

 

 





ICSI Videos


Video 1

Video 2

Video 3

 
(Please click on a link above, to open the video clip with the media player, supported by your browser. Alternatively, you may 'Right Click' on a link and choose an action.)





 

 

ICSI

 


 

Module 5 C


 

Module 5 D


 
 

Course objectives:

This course provides a one-on-one advanced training in high complexity techniques in a Laboratory. The syllabus covers the following areas:

Laboratory training:

  • Assisted hatching (chemical) - Learn how to perform assisted hatching using the chemical technique (Tyrode`s solution)
  • Embryo biopsy - Learn how to perform the embryo biopsy by removing one or more blastomeres. This technique is needed for laboratories which intend to perform pre-implantation genetic diagnosis
  • Embryo cryopreservation - Learn different protocols of embryo freezing , for either embryos with 8 or less cells or in blastocyst stage
  • ICSI with micro-HOS test - Learn how to perform the micro hypo osmotic swelling test for semen samples with no motility (practical)

Course duration:

Participants receive 5 days of instructions in the following areas:

  • Interactive discussions and observation - 10 hours
  • Laboratory training sessions - 30 hours
 

Course objectives:

This course provides customized training in the clinical management and laboratory practices of IVF and ICSI. Candidates can select the those techniques they wish to learn, the duration of their training, and whether they desire clinical observation in an IVF laboratory. The course duration may range from 1 week to 8 weeks depending on the candidates choice. Training is provided at a pace established by each candidate and is geared toward their personal needs. Candidates interested in this module should first contact our program to discuss interest.



 
 
lab

 
 

Hands on Assisted Reproduction (IVF/ICSI):


 
  ICSI
ICSI - Hatching - Blastocyst - Embryo
 
 
 
ICSI
Sperm - Egg - ICSI - Embryo
 
 
ACE

Training in this module
accredited by the
American College of Embryology –
Click on the logo for details

 

Educational teaching methods:

  • Live lectures and multimedia presentations
  • Training sessions using animal models (hamster oocytes/mouse embryos)
  • Training provided by trained embryologist, andrologist, reproductive endocrinologist and other experts in ART
  • Dedicated micromanipulators are available for training

Note:
Training offered in dedicated ART training suite inside a non clinical research laboratory. There is no patient contact.

Evaluation of trainee:

  • Knowledge: Multiple choice questions on lab techniques linked to each course module
  • Practical skills: Tested on specific lab techniques taught during the training
  • Verbal + communication skills: PPT talk on a topic linked to a course module

Each participant will receive:

  • Course syllabus with reading material and lab protocols
  • Free library access
  • Lunch tickets
  • Free Text Book on “Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices” Edited by Peter Nagy et al, Springer, Price $239.00 
  • WHO Laboratory Manual for the examination of human semen and sperm-cervical mucus interaction. Fifth Edition (CD)
  • Certificate of training (issued upon successful evaluation by the instructor)
     
Click here for a virtual 360 tour of the Center for Reproductive Medicine
Evaluation of ART training by Past Candidates
Click on this link to view the comments and feedback by previous ART Trainees.
Testimonials of Past Candidates
Click on this link to view the testimonials of previous ART Trainees.

Contact Information:

Training can be customized according to the needs of the candidate. Longer duration of training is available based on the interest and background of an individual candidate. Click here for information about various ART training modules and fee schedule. Click on ART Training Application for an online application. For more information contact:

Program Director
Center for Reproductive Medicine
Cleveland Clinic
9500 Euclid Avenue, A19
Cleveland, Ohio 44195, USA
Tel: 216-444-9485
Fax: 216-445-6049
E-mail: agarwaa@ccf.org

 

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Last Update : December 7, 2013
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Center for Reproductive Medicine
The Cleveland Clinic
9500 Euclid Avenue, Desk A19
Cleveland OH 44195
U.S.A.
CRM@ccf.org