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LABORATORY ANDROLOGY RESEARCH

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1.      PDF File (agradoc014.pdf   1168 Kb)
Ikemoto I, Fanning L, Loughlin KR, and Agarwal A (1994):
Sperm acrosin levels in semen: comparison between ACCU-SPERM and Kennedy's methods.
Andrologia 26:271-276.
 

2.      PDF File (agradoc021.pdf   821 Kb)
Prakash, P., Ozturk, E., MacLaughlin, D.T., Schiff, I., Loughlin, K.R., and Agarwal, A. (1995):
Interlaboratory, interprotocol comparison of indirect immunobead assay for sperm-associated antibodies in serum.
Arch Androl 34:133-139.
 

3.    PDF File (agradoc40.pdf 28 Kb)
Carbone, D.J., Jr., Seifarth, K, Thomas, A.J., Jr., and Agarwal, A. (1997):
Improved motile sperm recovery by a hyperosmotic Percoll gradient.
J Assist Reprod Genet 14:394-397.
 

4.      PDF File (agradoc047.pdf  1057 Kb)
Sharma, R.K., Seifarth, K., and Agarwal, A. (1997):
Comparison of single- and two-layer Percoll separation for selection of motile spermatozoa.
Int J Fertil Womens Med 42:412-417.
 

5.    PDF File (agradoc53.pdf 390 Kb)
Carbone, D.J.,Jr., McMahon, J., Levin, H.S., Thomas, A.J., Jr., and Agarwal, A. (1998):
Role of electron microscopy of sperm in the evaluation of male infertility during the era of assisted reproduction.
Urology 52:301-305.
 

6.
Esteves, S.C., Sharma, R.K., Thomas, A.J., Jr., and Agarwal, A. (1998):
The effects of sperm preparation by swim-up technique and capacitation on acrosome status of normozoospermic men.
Brazilian J Assist Reprod 2:74-82.
 

7.    PDF File (agradoc59.pdf 73 Kb)
Hendin, B.N., Patel, B., Levin, H.S., Thomas, A.J., Jr., and Agarwal, A. (1998):
Identification of spermatozoa and round spermatids in the ejaculates of men with spermatogenic failure.
Urology 51:816-819.
 

8.    PDF File (agradoc69.pdf 143 Kb)
Pasqualotto, E., Daitch, J., Hendin, B.N., Thomas, A.J., Jr., Nelson, D., and Agarwal, A. (1999):
Relationship of total motile sperm count and percent motile sperm to successful pregnancy rates following intrauterine insemination.
J Assist Reprod Genet 16:476-482.
 

9.    PDF File (agradoc70.pdf 58 Kb)
Sharma, R.K., Seifarth, K., Garlak, D., and Agarwal, A. (1999):
Comparison of three sperm preparation media.
Int J Fertil Womens Med 44:163-167.
 

10.    PDF File (agradoc86.pdf 46 Kb)
Esteves, S.C., Sharma, R.K., Thomas, A.J., Jr., and Agarwal, A. (2000):
Effect of swim-up sperm washing and subsequent capacitation on acrosome status and functional membrane integrity of normal sperm.
Int J Fertil Womens Med 45:335-341.
 

11.    PDF File (agradoc116.pdf  307 Kb)
Agarwal, A., Sharma, R.K., and Nelson, D.R. (2003):
New semen quality scores developed by principal component analysis of semen characteristics.
J Androl 24:343-352.
 

12.    PDF File (agradoc117.pdf  454 Kb)
Bedaiwy, M.A., Sharma, R.K., Alhussaini, T.K., Mohamed, M.S., Abdel-Aleem, A.M., Nelson, D.R., Thomas, A.J., Jr., and Agarwal, A. (2003):
The use of novel semen quality scores to predict pregnancy in couples with male-factor infertility undergoing intrauterine insemination.
J Androl 24:353-360.
 

13.    PDF File (agradoc151.pdf    133 Kb)
Allamaneni, SSR, Bandaranayake, I, Agarwal, A (2004):
Use of semen quality scores to predict pregnancy in couples undergoing intrauterine insemination with donor sperm.
Fertil Steril 82:606-611.
 

14.    PDF File (agradoc158.pdf    517 Kb)
Paasch, U, Sharma, RK, Gupta, AK, Grunewald, S, Mascha, EJ, Thomas, AJ, Jr, Glander, H-J, and Agarwal, A (2004):
Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa.
Biol Reprod 71:1828-1837.
 

15.    PDF File (agradoc160.pdf    198 Kb)
Zalata, AA, Ahmed, AH, Allamaneni, SSR, Comhaire, FH, Agarwal, A (2004):
Relationship between acrosin activity of human spermatozoa and oxidative stress.
Asian J Androl 6:313-318.
 

16.
Huang, Y, Agarwal, A (2004):
Determination and clinical significance of immature germ cells in human semen.
Zhonghua Nan Ke Xue 10: 290-292.
 

17.    PDF File (agradoc165.pdf    323 Kb)
Allamaneni, SSR, Agarwal, A, Rama, S, Ranganathan, P, and Sharma, RK (2005):
Comparative study on two density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa.
Asian J Androl 7:86-92.
 

18.    PDF File (agradoc172.pdf    640 Kb)
Said, TM, Grunewald, S, Paasch, U, Glander, HJ, Baumann, T, Kriegel, C, Li, L, and Agarwal, A (2005):
Advantage of combining magnetic cell separation with sperm preparation techniques.
Reprod Biomed Online 10:740-746.
 

19.    PDF File (agradoc177.pdf    149 Kb)
Said, TM, Grunewald, S, Paasch, U, Rasch, M, Agarwal, A, and Glander, HJ (2005):
Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates.
Fertil Steril 83:1442-1446.
 

20.    PDF File (agradoc179.pdf    335 Kb)
Said, TM, Agarwal, A, Falcone, T, Sharma, RK, Bedaiwy, MA, and Li, L (2005):
Infliximab may reverse the toxic effects induced by tumor necrosis factor alpha in human spermatozoa: an in vitro model.
Fertil Steril 83:1665-1673.
 

21.    PDF File (agradoc188.pdf    72 Kb)
Agarwal, A and Allamaneni, SSR (2005):
Sperm DNA damage assessment: a test whose time has come.
Fertil Steril 84:850-853.
 

22.    PDF File (agradoc204.pdf    254 Kb)
Said, TM, Agarwal, A, Grunewald, S, Rasch, M, Baumann, T, Kriegel, C, Li, L, Glander, H-J, Thomas, AJ, Jr, and Paasch, U (2005):
Selection of non-apoptotic spermatozoa as a new tool for enhancing assisted reproduction outcomes: an in-vitro model.
Biol Reprod 74:530-537.
 

23.    PDF File (agradoc208.pdf    191 Kb)
Grunewald, S, Paasch, U, Said, TM, Rasch, M, Agarwal, A, and Glander, H-J (2006):
Magnetic-activated cell sorting before cryopreservation preserves mitochondrial integrity in human spermatozoa.
Cell Tissue Bank 7:99-104.
 

24.    PDF File (agradoc214.pdf    120 Kb)
Kumanov, P, Nandipati, K, Tomova, A, Agarwal, A (2006):
Inhibin B is a better marker of spermatogenesis than other hormones in the evaluation of male factor infertility.
Fertil Steril 86:332-338.
 

25.    PDF File (agradoc223.pdf    80 Kb)
Gupta, S, Banerjee, J, Agarwal, A (2006):
The impact of reactive oxygen species on early human embryos. A systematic review of the literature.
Embryo Talk 1.2:87-98.
 

26.    PDF File (agradoc225.pdf    255 Kb)
Said, TM, Agarwal, A, Grunewald, S, Rasch, M, Glander, H-J, and Paasch, U (2006):
Evaluation of sperm recovery following annexin-V MACS separation. Review Article.
Reprod BioMed Online 13:336-339.
 

27.    PDF File (agradoc232.pdf    31 Kb)
Alvarez, JG and Agarwal, A (2006):
Letter to the editor: Development of a novel home sperm test- What are the limitations?
Hum Reprod 21:3029-3030.
 

28.    PDF File (agradoc233.pdf    68 Kb)
Prabakaran, S, Agarwal, A, Sundaram, A, Thomas, AJ Jr., Sikka, S (2006):
Cryosurvival of testicular spermatozoa from obstructive azoospermic patients: The Cleveland Clinic experience.
Fertil Steril 86:1789-1791.
 

29.    PDF File (agradoc230.pdf    413 Kb)
Agarwal, A, Sharma, RK (2006):
Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer.
Fertil Steril 87:156-162. [E-pub]
 

30.    PDF File (agradoc235.pdf    356 Kb)
Aziz, N, Said, T, Paasch, U, Agarwal, A (2007):
The relationship between sperm apoptosis, morphology and the sperm deformity index.
Human Reproduction 22: 1413-1419.
 

31.    PDF File (agradoc238.pdf    213 Kb)
Agarwal, A, Deepinder, F, Cocuzza, M, Short, RA, Evenson, DP (2007):
Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study.
Fertil Steril. 2008: 89:375-379.
 

32.    PDF File (agradoc242.pdf    424 Kb)
Deepinder, F, Chowdary, H, Agarwal, A, (2007):
Role of metabolomic analysis biomarkers in the management of male infertility.
Expert Review of Molecular Diagnostics 7:351-358.
 

33.    PDF File (agradoc243.pdf    148 Kb)
Esteves, SC, Sharma, RK, Thomas, AJ, Agarwal, A (2007):
Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test.
Int Braz J Urol. 33:364-74; Discussion 375-376.
 

34.    PDF File (agradoc245.pdf    1215 Kb)
Athayde, KS, Cocuzza, M, Hallak, J, Agarwal, A, Lucon, AM, Krajcir, N, Srougi, M. (2007):
Development of normal reference values for seminal reactive oxygen species and their correlation with leukocytes and semen parameters in a fertile population: a prospective controlled study.
J Androl. 28:613-620.
 

35.    PDF File (agradoc253.pdf    101 Kb)
Bedaiwy, M, Agarwal, A, Said, TM, Goldberg, JM, Sharma, RK, Worley, S, and Falcone, T (2006):
Role of total antioxidant capacity in the differential growth of human embryos in vitro.
Fertil Steril 86: 304-309.
 

36.    PDF File (agradoc260.pdf    55 Kb)
Aziz, N. and Agarwal, A. (2007):
Evaluation of sperm damage: Beyond the WHO criteria. Commentary. Review Article.
Fertil Steril. 2007 Oct 20; [Epub ahead of print].
 

37.    PDF File (agradoc271.pdf    474 Kb)
Said, M, Agarwal, A, Glander, HJ, Grunewald, S, Zborowski, M, Paasch, U, (2008):
Utility of magnetic cell separation as a molecular sperm separation technique.
J Androl 29:134-42.
 

38.
Abdelrazik, H, Sharma, R., Mahfouz, R, Agarwal, A (2008):
L Carnitine (LC) decreases DNA damage and improves the in vitro blastocyst development rate in mouse embryos.
Fertil Steril Feb 2; [E-pub ahead of print].
 

39.    PDF File (agradoc305.pdf    357 Kb)   new
Mukhopadhyay D, Varghese AC, Pal M, Banerjee SK, Bhattacharyya AK, Sharma RK, Agarwal A (2009):
Semen quality and age-specific changes: A study between two decades on 3729 male partners of couples with normal sperm count and attending an andrology laboratory for infertility-related problems in an Indian city.
Fertil Steril. [E-pub]
 

40.    PDF File (agradoc320.pdf    94 Kb)   new
Mahfouz, R, Said, TM, Agarwal, A (2009):
The diagnostic and therapeutic applications of flow cytometry in male infertility. Review Article.
Arch of Med Sci. 5, 1A: S99-S108.
 

41.    PDF File (agradoc301.pdf    196 Kb)   new
Agarwal A, Desai N, Makker K, Varghese A, Mouradi M, Sabanegh E, Sharma R (2008):
Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: An in vitro pilot study.
Fertil Steril [E-pub]
 

42.    PDF File (agradoc331.pdf    954 Kb)   new
Agarwal A (2009):
Guest editorial: What is in the future of sperm analysis?
Journal of Clinic. Embryo. Vol 12: 2.
 

43.    PDF File (agradoc332.pdf    530 Kb)   new
Mahfouz R, Sharma R, Lackner J, Aziz N, Agarwal A (2009):
Evaluation of chemiluminescence and flow cytometry as tools in assessing production of hydrogen peroxide and superoxide anion in human spermatozoa.
Fertil Steril; 92(2): 819-827.
 

44.    PDF File (agradoc333.pdf    44 Kb)   new
Mahfouz R, Sharma R, Lackner J, Aziz N, Agarwal A (2009):
Letter to the editor.
Fertil steril; 92(2): e20
 

 
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