Jennifer Perpar Cellular Signaling in Human Heart Failure
SchoolLake Catholic High School
MentorWendy E. Sweet, MS
DepartmentKaufman Center for Heart Failure
Cellular Signaling in Human Heart Failure
Glycogen synthase kinase-3ß (GSK-3ß) is an important inhibitor in many pathways that lead to hypertrophy. Phosphorylation of GSK-3ß (pGSK-3ß) inactivates the protein’s ability to inhibit these pathways. We hypothesize that pGSK-3ß is increased in human heart failure and its expression is greater in females.
pGSK-3ß and GSK-3ß were measured by Western blotting in non-failing hearts (NF) and hearts with dilated cardiomyopathy (DCM).
While pGSK-3ß protein levels were significantly decreased in DCM hearts compared to NF hearts (p < 0.001), no difference in GSK-3ß was found. No changes in the expression of pGSK-3ß or GSK-3ß were identified based on gender. The lack of pGSK-3ß in end-stage heart failure in patients with DCM may be key to the dilation and thin walls found in these hearts.