Jennifer Perpar
Cellular Signaling in Human Heart Failure

SchoolLake Catholic High School


ProgramScience


MentorWendy E. Sweet, MS


DepartmentKaufman Center for Heart Failure


Research
Cellular Signaling in Human Heart Failure
Hypothesis
Glycogen synthase kinase-3ß (GSK-3ß) is an important inhibitor in many pathways that lead to hypertrophy. Phosphorylation of GSK-3ß (pGSK-3ß) inactivates the protein’s ability to inhibit these pathways. We hypothesize that pGSK-3ß is increased in human heart failure and its expression is greater in females.
Methodology
pGSK-3ß and GSK-3ß were measured by Western blotting in non-failing hearts (NF) and hearts with dilated cardiomyopathy (DCM).
Outcomes
While pGSK-3ß protein levels were significantly decreased in DCM hearts compared to NF hearts (p < 0.001), no difference in GSK-3ß was found. No changes in the expression of pGSK-3ß or GSK-3ß were identified based on gender. The lack of pGSK-3ß in end-stage heart failure in patients with DCM may be key to the dilation and thin walls found in these hearts.

Interpretations

Heart by Kimberly Sutadji