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CRYOBIOLOGY OF HUMAN SPERMATOZOA

1.    PDF File (agradoc27.pdf 444 Kb)
Sharma, R.K., Tolentino, M.V.,Jr., and Agarwal, A. (1996):
Sperm kinematics of cryopreserved normozoospermic specimens after artificial stimulation.
Urology 47:77-81.
 

2.      PDF File (agradoc030.pdf  1321 Kb)
Esteves, S.C., Sharma, R.K., Thomas, A.J., Jr., and Agarwal, A. (1996):
Suitability of the hypo-osmotic swelling test for assessing the viability of cryopreserved sperm.
Fertil Steril 66:798-804.
 

3.      PDF File (agradoc026.pdf   1085 Kb)
Sharma RK, Tolentino MV,Jr., Thomas AJ Jr, and Agarwal A (1996):
Optimal dose and duration of exposure to artificial stimulants in cryopreserved human spermatozoa.
J Urol 155:568-573.
 

4.      PDF File (agradoc028.pdf   1235 Kb)
Sharma RK and Agarwal A (1996):
Sperm quality improvement in cryopreserved human semen.
J Urol 156:1008-1012.
 

5.      PDF File (agradoc033.pdf  848 Kb)
Sharma, R.K. and Agarwal, A. (1997):
Influence of artificial stimulation on unprocessed and Percoll-washed cryopreserved sperm.
Arch Androl 38:173-179.
 

6.      PDF File (agradoc034.pdf  1212 Kb)
Sharma RK, Padron OF, and Agarwal A (1997):
Usefulness of the acrobead test in evaluating human acrosome function in fresh and cryopreserved sperm.
J Urol 157:1692-1696.
 

7.      PDF File (agradoc037.pdf  704 Kb)
Sharma, R.K., Vemulapalli, S., Kohn, S., and Agarwal, A. (1997):
Effect of centrifuge speed, refrigeration medium, and sperm washing medium on cryopreserved sperm quality after thawing.
Arch Androl 39:33-38.
 

8.      PDF File (agradoc041.pdf  3321 Kb)
Sharma, R.K. and Agarwal, A. (1997):
Artificial stimulation of cryopreserved human spermatozoa by sodium nitroprusside, 2-chloroadenosine and 2-deoxyadenosoine.
Eur Urol 32:344-352.
 

9.    PDF File (agradoc42.pdf 603 Kb)
Sharma, R.K., Padron, O.F., Thomas, A.J., Jr., and Agarwal, A. (1997):
Factors associated with the quality before freezing and after thawing of sperm obtained by microsurgical epididymal aspiration.
Fertil Steril 68:626-631.
 

10.    PDF File (agradoc46.pdf 449 Kb)
Sidhu, R.S., Sharma, R.K., and Agarwal, A. (1997):
Effects of cryopreserved semen quality and timed intrauterine insemination on pregnancy rate and gender of offspring in a donor insemination program.
J Assist Reprod Genet 14:531-537.
 

11.    PDF File (agradoc48.pdf 546 Kb)
Sidhu, R.S., Sharma, R.K., Lee, J-C., and Agarwal, A. (1998):
Accuracy of computer-assisted semen analysis in pre-freeze and post-thaw specimens with high and low sperm counts and motility.
Urology 51:306-312.
 

12.    PDF File (agradoc57.pdf 81 Kb)
Esteves, S.C., Sharma, R.K., Thomas, A.J., Jr., and Agarwal, A. (1998):
Cryopreservation of human spermatozoa with pentoxifylline improves the post-thaw agonist-induced acrosome reaction rate.
Hum Reprod 13:3384-3389.
 

13.    PDF File (agradoc58.pdf 38 Kb)
Esteves, S.C., Sharma, R.K., Thomas, A.J., Jr., and Agarwal, A. (1998):
Effect of in vitro incubation on spontaneous acrosome reaction in fresh and cryopreserved human spermatozoa.
Int J Fertil Womens Med 43:235-242.
 

14.    PDF File (ladoc22.pdf 22 Kb)
Esteves, S.C., Sharma, R.K., Thomas, A.J., Jr., and Agarwal, A. (1999):
Sperm viability assays - a matter of life and death [letter; comment].
Fertil Steril 72:184-185.
 

15.    PDF File (agradoc77.pdf 36 Kb)
Hallak, J., Sharma, R.K., Wellstead, C. and Agarwal, A. (2000):
Cryopreservation of human spermatozoa: comparison of TEST-yolk buffer and glycerol.
Int J Fertil Womens Med 45:38-42.
 

16.    PDF File (agradoc85.pdf 121 Kb)
Esteves, S.C., Sharma, R.K., Thomas, A.J., Jr., and Agarwal, A. (2000):
Improvement in motion characteristics and acrosome status in cryopreserved human spermatozoa by swim-up processing before freezing.
Hum Reprod 15:2173-2179.
 

17.    PDF File (agradoc154.pdf 216 Kb)
Nallella, KP, Sharma, RK, Allamaneni, SSR, Aziz, N, and Agarwal, A (2004):
Cryopreservation of human spermatozoa: comparison of two cryopreservation methods and three cryoprotectants.
Fertil Steril 82:913-918.
 

18.    PDF File (agradoc158.pdf 517 Kb)
Paasch, U, Sharma, RK, Gupta, AK, Grunewald, S, Mascha, EJ, Thomas, AJ, Jr, Glander, HJ, and Agarwal, A (2004):
Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa.
Biol Reprod 71:1828-1837.
 

19.    PDF File (agradoc177.pdf 149 Kb)
Said, TM, Grunewald, S, Paasch, U, Rasch, M, Agarwal, A, and Glander, H-J (2005):
Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates.
Fertil Steril 83:1442-1446.
 

20.    PDF File (agradoc208.pdf 191 Kb)
Grunewald, S, Paasch, U, Said, TM, Rasch, M, Agarwal, A, and Glander, H-J (2006):
Magnetic-activated cell sorting before cryopreservation preserves mitochondrial integrity in human spermatozoa.
Cell Tissue Bank 7:99-104.
 

21.    PDF File (agradoc233.pdf 68 Kb)
Prabakaran, S, Agarwal, A, Sundaram, A, Thomas, AJ Jr., Sikka, S (2006):
Cryosurvival of testicular spermatozoa from obstructive azoospermic patients: The Cleveland Clinic experience.
Fertil Steril 86:1789-1791.
 

22.    PDF File (agradoc380.pdf 148 Kb)
Agarwal A, Burns WR, Dada R, Sabanegh E (2010):
Male infertility and testicular cancer: points of common causality. Review Article.
Eur Urol Rev 5(1): 5659.
 

23.    PDF File (agradoc390.pdf 171 Kb)
Gupta S, Agarwal A, Sharma R, Ahmady A (2010):
Recovery, preparation, storage and utilization of spermatozoa for fertility preservation in cancer patients and sub-fertile men. Review Article.
J Reprod Stem Cell Biotechnol 1(2): 150-168.
 

 
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Last Update : February 07, 2013
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